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M94A3235.TXT
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1994-10-25
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Document 3235
DOCN M94A3235
TI Monomeric HIV-gp120 induces both inhibition of cell proliferation and
apoptosis.
DT 9412
AU Tuosto L; Lorenzetti S; Cundari E; Moretti S; Lombardi G; Piccolella E;
Dept. Cell. and Develop. Biology, Univ. of Rome, Italy.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):122 (abstract no. PA0106). Unique
Identifier : AIDSLINE ICA10/94369340
AB Infection with human immunodeficiency virus (HIV) results in a
progressive impairment of immune responses, partly due to the defect
induced in T cells following the interaction of the HIV-envelope
glycoprotein (gp120) and/or anti-CD4 antibodies with CD4 molecules.
These effects have been reported as inhibition of mitogen- and
antigen-induced T cell proliferation, and induction of apoptosis.
However, it is not clear if cross-linking of CD4 molecules by HIV gp120
or anti-CD4 antibodies is required to induce either the impairment of
cell proliferation or the induction of apoptosis in antigen-primed
lymphocytes. To this aim we have analyzed the effect of monomeric gp120
and anti-CD4 antibodies on proliferative response and
apoptosis-induction on a panel of antigen-specific T cell clones and
freshly isolated CD4+ T lymphocytes. We found new experimental
conditions able to induce apoptosis by involvement of CD4 molecules
without cross-linking of HIV gp120 and CD4 antibodies. Our results
clearly demonstrate that: a) the need for CD4 cross-linking is
restricted only to fresh purified CD4+ lymphocytes stimulated by
anti-CD3 antibody, while antigen-specific T cell clones are sensible to
programmed cell death mediated by monomeric gp120; b) soluble anti-CD4
antibodies are able to induce apoptosis in both fresh T lymphocytes and
antigen-specific T cell clones. Our results suggest that during HIV
infection anti-CD4 antibodies and gp120 could contribute to the
unresponsiveness state and programmed cell death of CD4+ uninfected T
lymphocytes during an antigen-stimulation.
DE Antibodies/PHARMACOLOGY Antigens, CD/IMMUNOLOGY/PHYSIOLOGY Antigens,
CD4/IMMUNOLOGY/PHYSIOLOGY *Apoptosis Cell Division Clone Cells
Comparative Study Human HIV/*PHYSIOLOGY HIV Envelope Protein
gp120/IMMUNOLOGY/*METABOLISM In Vitro *Lymphocyte Transformation
T-Lymphocytes/CYTOLOGY/IMMUNOLOGY/*MICROBIOLOGY T4
Lymphocytes/CYTOLOGY/IMMUNOLOGY/*MICROBIOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).